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proJ [2018-02-02T15:57:43.000Z]
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proJ [2018-02-02T15:57:43.000Z]

glutamate 5-kinase
Locus
BSU18470
Isoelectric point
5.30
Molecular weight
40.20 kDa
Protein length
371 aa Sequence Blast
Gene length
1113 bp Sequence Blast
Function
osmoadaptive de novo production of proline
Product
glutamate 5-kinase
Essential
no
E.C.
2.7.2.11
Synonyms
yohA

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,015,733 → 2,016,848

The protein

Catalyzed reaction/ biological activity

  • ATP L-glutamate = ADP L-glutamate 5-phosphate (according to Swiss-Prot)
  • Paralogous protein(s)

    Structure

  • 4Q1T (from Burkholderia thailandensis, 39% identity)
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulation

  • expressed under conditions of osmotic stress PubMed
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    Biological materials

    Mutant

  • GP817 (spc) available in the Stülke lab
  • 1A635 ( proJ::erm), PubMed, available at BGSC
  • BKE18470 (ΔproJ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TCTTTTCATGCTTGTATCAG, downstream forward: _UP4_TAATTTGCAGAAAAAAACCC
  • BKK18470 (ΔproJ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TCTTTTCATGCTTGTATCAG, downstream forward: _UP4_TAATTTGCAGAAAAAAACCC
  • References

    Reviews

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    Original publications

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